Programming gene expression in multicellular organisms for physiology modulation through engineered bacteria

Abstract A central goal of synthetic biology is to predictably and efficiently reprogram living systems perform computations carry out specific biological tasks. Although there have been many advances in the bio-computational design systems, these mainly applied microorganisms or cell lines; programming animal physiology remains challenging for because system complexity. Here, we present a bacteria-animal symbiont which engineered bacteria recognize external signals modulate gene expression, twitching phenotype, fat metabolism through RNA interference toward gfp , sbp-1 unc-22 C. elegans . By using genetic circuits control expressions, are able program model Caenorhabditis with logic gates. We anticipate that can be used more extensively agricultural, therapeutic, basic science applications.